http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/134 2026-04-07T14:09:37Z 2026-04-07T14:09:37Z Rico Fuentes, Cecilia López Pulido, Edgar Iván Pérez Guerrero, Edsaúl Emilio Godínez Rubí, Marisol Villegas Pineda, Julio César Villanueva Pérez, Martha Arisbeth Villanueva-Pérez Sierra Díaz, Erick Zepeda Nuño, José Sergio Pereira Suárez, Ana Laura Ramírez de Arellano, Adrián http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1607 2024-02-15T21:38:50Z 2024-02-01T00:00:00Z

Title: Positive correlation between the nuclear expression of GPER and pGLI3 in prostate cancer tissues from patients with different Gleason scores Authors: Rico Fuentes, Cecilia; López Pulido, Edgar Iván; Pérez Guerrero, Edsaúl Emilio; Godínez Rubí, Marisol; Villegas Pineda, Julio César; Villanueva Pérez, Martha Arisbeth Villanueva-Pérez; Sierra Díaz, Erick; Zepeda Nuño, José Sergio; Pereira Suárez, Ana Laura; Ramírez de Arellano, Adrián Abstract: Prostate cancer (PCa) is the most prevalent cause of death in the male population worldwide. The G Protein-Coupled Estrogen Receptor (GPER) has been gaining relevance in the development of PCa. Hedgehog (Hh) pathway activation is associated with aggressiveness, metastasis, and relapse in PCa patients. To date, no studies have evaluated the crosstalk between the GPER and the Hh pathway along different group grades in PCa. We conducted an analysis of paraffin-embedded tissues derived from patients with different prognostic grade of PCa using immunohistochemistry. Expression and correlation between GPER and glioma associated oncogene homologue (GLI) transcriptional factors in the parenchyma and stroma of PCa tumors were evaluated. Our results indicate that GPER is highly expressed in the nucleus and increases with higher grade groups. Additionally, GPER’s expression correlates with pGLI3 nuclear expression across different grade groups in PCa tissues; however, whether the receptor induces the activation of GLI transcriptional factors, or the latter modulate the expression of GPER is yet to be discovered, as well as the functional consequence of this correlation. Description: Artículo

2024-02-01T00:00:00Z Bravo Cuéllar, Alejandro Lerma Díaz, José Manuel http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/919 2019-11-22T19:44:40Z 2009-01-01T00:00:00Z

Title: Apoptosis Authors: Bravo Cuéllar, Alejandro; Lerma Díaz, José Manuel Abstract: Detection of apoptotic cell death in cells and tissues has become of crucial importance in many fields of modern Biology, including studies of embryonic development, degenerative diseases, and Cancer Biology. The need for reliable methods to evaluate death of cells, especially in clinical and experimental Oncology, has led to the development of several techniques. Most of these methods take advantage of properties of dying cells that are more or less specific for the apoptotic process. However, considerable debate exists over the interpretation of some of these methods and their usefulness in all settings. There are many ways of detecting apoptosis and the methods used in the laboratories are mainly based on the identification of apoptotic cell populations by epifluorescence microscopy, flow cytometry and DNA laddering assay. Here we discuss some of the practical utility of such techniques according to our experience.

2009-01-01T00:00:00Z Gómez Rodríguez, Víctor Manuel Rodríguez Garay, Benjamín Palomino, Guadalupe Martínez, Javier Barba González, Rodrigo http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/555 2016-09-21T15:08:07Z 2013-08-12T00:00:00Z

Title: Physical mapping of 5S and 18S ribosomal DNA in three species of Agave (Asparagales, Asparagaceae) Authors: Gómez Rodríguez, Víctor Manuel; Rodríguez Garay, Benjamín; Palomino, Guadalupe; Martínez, Javier; Barba González, Rodrigo Abstract: Agave Linnaeus, 1753 is endemic of America and is considered one of the most important crops in Mexico due to its key role in the country’s economy. Cytogenetic analysis was carried out in A. tequilana Weber, 1902 ‘Azul’, A. cupreata Trelease et Berger, 1915 and A. angustifolia Haworth, 1812. The analysis showed that in all species the diploid chromosome number was 2n = 60, with bimodal karyotypes composed of five pairs of large chromosomes and 25 pairs of small chromosomes. Furthermore, different karyotypical formulae as well as a secondary constriction in a large chromosome pair were found in all species. Fluorescent in situ hybridization (FISH) was used for physical mapping of 5S and 18S ribosomal DNA (rDNA). All species analyzed showed that 5S rDNA was located in both arms of a small chromosome pair, while 18S rDNA was associated with the secondary constriction of a large chromosome pair. Data of FISH analysis provides new information about the position and number of rDNA loci and helps for detection of hybrids in breeding programs as well as evolutionary studies.

2013-08-12T00:00:00Z Gómez Rodríguez, Víctor Manuel Rodríguez Garay, Benjamín Barba González, Rodrigo http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/554 2022-10-19T16:46:46Z 2012-09-01T00:00:00Z

Title: Meiotic restitution mechanisms involved in the formation of 2n pollen in Agave tequilana Weber and Agave angustifolia Haw Authors: Gómez Rodríguez, Víctor Manuel; Rodríguez Garay, Benjamín; Barba González, Rodrigo Abstract: A cytological analysis of the microsporogenesis was carried out in the Agave tequilana and A. angustifolia species. Several abnormalities such as chromosomal bridges, lagging chromosomes, micronuclei, monads, dyads and triads were found. The morphological analysis of the pollen, together with the above-mentioned 2n microspores, allowed us to confirm the presence of 2n pollen as well as its frequency. In both A. tequilana and A. angustifolia two different mechanisms were observed: the first mechanism, a failure in the cytokinesis in meiosis II caused the formation of dyads with two 2n cells and triads containing two n cells and one 2n cell; the second mechanism, involves an abnormal spindle, which caused the formation of triads with two n cells and one 2n cell. Likewise, the presence of monads was detected in both species, these, might be caused by a failure of the cytokinesis in both meiotic divisions. This is the first report about the presence of a Second Division Restitution mechanism (SDR) which causes the formation of 2n pollen in the genus Agave. The genetic implications of the presence of 2n pollen in the genus Agave are discussed.

2012-09-01T00:00:00Z