http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/38 2026-03-12T19:46:56Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1765 Title: Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis Authors: Escoto Vasquez, Lilibeth Stephania; Alarcón Sánchez, Mario Alberto; Becerra Ruiz, Julieta Sarai; Martínez Bugarin, Cristina Hermila; Lomelí Martínez, Sarah Monserrat; Muradyan, Armen A.; Heboyan, Artak Abstract: Objective This project aimed to evaluate the immunoexpression pattern of murine double minute 2 (MDM2) in solid ameloblastomas compared to unicystic ameloblastomas. Methods The review followed PRISMA guidelines and was registered in the PROSPERO database. PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar were comprehensively searched. Original cross-sectional studies were included. The meta-analysis was performed using STATA V15 and RevMan. Positivity rates were pooled using a random-effects model (REM), the labeling index was analyzed using mean difference under a REM, and expression intensity (moderate–strong) was assessed as categorical data using a REM with Hartung-Knapp adjustment. Heterogeneity was evaluated with the Chi² test and I² statistic. The methodological quality and certainty of the evidence were assessed using the Joanna Briggs Institute items and the GRADE system. Results Nine studies (n = 438 specimens) were analyzed, of which 325/438 (74.2 %) were ameloblastoma biopsies. MDM2 positivity was detected in 403/438 cases (92 %). A statistically significant association in favor of solid ameloblastoma was observed (RR = 2.08; 95 %CI [1.66–2.60]; p = 0.005), indicating a twofold probability of finding high MDM2 expression in solid compared to unicystic ameloblastomas. Four of the nine studies (44.4 %) were considered to be of low quality, and the certainty of evidence was low to very low. Conclusions MDM2 expression was prevalent in both types of ameloblastomas, with a higher intensity of expression observed in solid cases. However, due to study heterogeneity, further investigations with more robust methodological designs are recommended to assess the diagnostic potential of MDM2 in ameloblastomas. Description: Artículo 2025-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1760 Title: Association of IL32 Variant rs45499297 with Gingivitis and IL-32 Levels: A Cross-sectional Clinical-bioinformatics Study Authors: Vázquez Jiménez, Sonia Isela; Martínez Perez, Luz Andrea; Becerra Ruiz, Julieta Saraí; González Sánchez, Grecia Denisse; Perez Reyes, Angel; Martínez Esquivias, Fernando; Guzmán Flores, Juan Manuel Abstract: Introduction: Gingivitis is the initial manifestation of periodontal disease, characterized by bacteria and inflammatory mediators, such as IL-32. This study aimed to determine whether the rs45499297 genetic variant of the IL32 gene and its serum and salivary levels are associated with gingivitis. In addition, we investigated whether transcription factors bind differentially in the presence of this genetic variant. Methods: The study was conducted with 147 subjects. The rs45499297 variant was analyzed using the PCR-RFLP technique, while cytokine levels were measured using the ELISA assay. The affinity of the transcription factors in the presence of the gene variant was analyzed using bioinformatics methods. The results suggest that schooling, salivary flow, lipids, and salivary IL-32 levels were associated with gingivitis. Furthermore, the TC genotype, in the presence of obesity, conferred an increased risk of gingivitis, which was associated with lower serum IL-32 levels. Bioinformatics analysis revealed that the transcriptional factor LRH1 binds with a higher affinity to the C allele than to the T allele of the studied genetic variant. Results: The findings of this study demonstrate the multifactorial nature of gingivitis, wherein the interplay between genetics and obesity serves as a regulatory factor in inflammation and periodontal risk. The preferential binding of LRH1 to the C allele suggests a molecular link between the genetic variant, inflammatory dysregulation, and the altered metabolic environment in obesity. Conclusion: Our data show that the rs45499297 gene variant is only associated with gingivitis in the presence of obesity and affects serum cytokine levels. Additionally, we identified the potential involvement of LRH1 in regulating the IL-32 gene expression. Description: Artículo 2025-10-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1759 Title: Hepatitis and periodontal health: an emerging oral-liver axis Authors: Rodríguez Montaño, Ruth; Martínez Nieto, Melissa; González Alvarez, Gustavo Eder; Alarcó Sánchez, Mario Alberto; Becerra Ruiz, Julieta Sarai; Heboyan, Artak; Ruiz Gaitán, Alba; Lomelí Martínez, Sarah Monserrat Abstract: Periodontitis and viral liver infections, particularly hepatitis B virus (HBV) and hepatitis C virus (HCV), are chronic inflammatory conditions with a high prevalence worldwide. Recent evidence establishes a possible bidirectional relationship between the two, based on shared immunological, microbial, and inflammatory mechanisms. The objective of this study was to analyze and synthesize the scientific literature on the interactions between viral hepatitis and periodontal health. Through a structured search of the PubMed, Scopus, and Web of Science databases, studies published in the last 20 years that explored the link between viral hepatitis and periodontitis were integrated. The findings from the reviewed studies show consistent, positive associations between HBV and HCV viruses and a higher prevalence and severity of periodontitis. Some studies show increased levels of proinflammatory cytokines (such as IL-6 and TNF-α) and immune dysfunction in participants with both diseases. Additionally, viral markers (such as HBsAg and HCV RNA) have been identified in gingival crevicular fluid, suggesting the presence of oral viral reservoirs. Ultimately, scientific evidence suggests a bidirectional relationship between viral hepatitis and periodontitis, influenced by systemic inflammation, immunological alterations, and microbial dysbiosis. The collected data support the relevance of interdisciplinary management between medical and dental professionals in patients with viral liver conditions. Description: Artículo 2025-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1758 Title: The Role of Interleukin-23 and Interleukin-17 in Peri-Implant Crevicular Fluid of Subjects With Peri-Implant Disease: A Systematic Review Authors: Alarcón Sánchez, Mario Alberto; Becerra Ruiz, Julieta Sarai; Rodríguez Montaño, Ruth; Lomelí Martínez, Sarah Monserrat; Escoto Vasquez, Lilibeth Stephania; Heboyan, Artak Abstract: Background Activation of the IL-23/IL-17 cytokine axis could trigger peri-implant bone loss. The aim of this review was to analyze whether in people with peri-implantitis (PI) and peri-implant mucositis (PM) the concentrations of the interleukin-23 and interleukin-17 in peri-implant crevicular fluid (PICF) are elevated compared to people with healthy dental implants (HDI). Methodology The protocol of this study was registered in OSF (ID: 10.17605/OSF.IO/U8NBQ) and followed PRISMA guidelines. PECO criteria were used to formulate the research question. A search strategy was performed using PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar until November 15, 2024. A rigorous evaluation was performed, and the JBI tool was used to assess the quality of the cross-sectional and case–control studies. Results Fourteen observational studies were included in this study, with a total of 587 participants carrying 601 dental implants. The control group was represented by 252 healthy implants, while the exposure group was represented by 113 implants with PM and 236 implants with peri-implantitis. The age range of the subjects varied from 40.8 to 68.6 years, with a mean age ± standard deviation of 53.9 ± 9.9 years. The concentration of the IL-23/IL-17 cytokine axis and isoforms (IL-17E and IL-17F) was higher in subjects with peri-implant disease compared to the healthy population. Most of the studies (92.8%) showed moderate quality. Conclusions The concentrations of cytokines IL-23, IL-17, and IL-17E in PICF were higher in PI-affected dental implants, followed by PM-affected dental implants compared to HDI. Description: Artículo 2025-08-01T00:00:00Z