http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1125 Fri, 23 Jan 2026 09:44:07 GMT 2026-01-23T09:44:07Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1631 Title: Pharmacological network study on the effect of 6-gingerol on cervical cancer using computerized databases Authors: Martínez Esquivias, Fernando; Guzmán Flores, Juan Manuel; Chávez Díaz, Ismael Fernando; Iñiguez Muñoz, Laura Elena; Reyes Chaparro, Andrés Abstract: Cervical cancer (CC) is the most frequent cancer in the female population worldwide. Although there are treatments available, they are ineffective and cause adverse effects. 6-gingerol is an active component in ginger with anticancer activity. This research aims to discover the mechanism by which 6-gingerol act as an anticancer agent on CC through a pharmacological network using bioinformatics databases. From MalaCard, Swiss Target Prediction, Comparative Toxicogenomics Database, and Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, we obtained the target genes for 6-gingerol and CC and matched them. We got 26 genes and analyzed them in ShinyGO-0.76.3 and DAVID-Bioinformatics Resources. Then, we generated a protein-protein interaction network in Cytoscape and obtained 12 hub genes. Hub genes were analyzed in Gene Expression Profiling Interactive Analysis and TISIDB. In addition, molecular docking studies were performed between target proteins with 6-gingerol using SwissDock database. Finally, molecular dynamics studies for three proteins with the lowest interaction energy were implemented using Gromacs software. According to gene ontology results, 6-gingerol is involved in processes of apoptosis, cell cycle, and protein kinase complexes, affecting mitochondria and pathways related to HPV infection. CTNNB1 gene was negatively correlated with CD8þ infiltration but was not associated with a higher survival rate. Furthermore, the molecular docking study showed that 6-gingerol has a high binding to proteins, and the molecular dynamics showed a stable interaction of 6-gingerol to AKT1, CCNB1, and CTNNB1 proteins. Conclusion, our work helps to understand the anticancer activity of 6-gingerol in CC that should be studied experimentally. Description: Artículo Fri, 01 Sep 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1631 2023-09-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1627 Title: Evaluation of the Biological Properties of an Optimized Extract of Polygonum cuspidatum Using Ultrasonic-Assisted Extraction Authors: Fletes Vargas, Gabriela; Rodríguez Rodríguez, Rogelio; Pacheco, Neith; Pérez Larios, Alejandro; Espinosa Andrews, Hugo Abstract: Phytochemicals are natural compounds found in plants that have potential health benefits such as antioxidants, anti-inflammatory and anti-cancer properties, and immune reinforcement. Polygonum cuspidatum Sieb. et Zucc. is a source rich in resveratrol, traditionally consumed as an infusion. In this study, P. cuspidatum root extraction conditions were optimized to increase antioxidant capacity (DPPH, ABTS+), extraction yield, resveratrol concentration, and total polyphenolic compounds (TPC) via ultrasonic-assisted extraction using a Box–Behnken design (BBD). The biological activities of the optimized extract and the infusion were compared. The optimized extract was obtained using a solvent/root powder ratio of 4, 60% ethanol concentration, and 60% ultrasonic power. The optimized extract showed higher biological activities than the infusion. The optimized extract contained 16.6 mg mL−1 resveratrol, high antioxidant activities (135.1 µg TE mL−1 for DPPH, and 230.4 µg TE mL−1 for ABTS+), TPC (33.2 mg GAE mL−1), and extraction yield of 12.4%. The EC50 value (effective concentration 50) of the optimized extract was 0.194 µg mL−1, which revealed high cytotoxic activity against the Caco-2 cell line. The optimized extract could be used to develop functional beverages with high antioxidant capacity, antioxidants for edible oils, functional foods, and cosmetics. Description: Artículo Mon, 01 May 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1627 2023-05-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1613 Title: Long chain capsaicin analogues synthetized by CALB‑CLEAs show cytotoxicity on glioblastoma cell lines Authors: Diaz Vidal, Tania; Armenta Pérez, Vicente Paúl; Rosales Rivera, Luis Carlos; Basulto Padilla, Georgina Cristina; Martínez Pérez, Raúl Balam; Mateos Díaz, Juan Carlos; Gutiérrez Mercado, Yanet Karina; Canales Aguirre, Alejandro A.; Rodríguez, Jorge A. Abstract: Abstract Glioblastoma is one of the most lethal tumors, displaying striking cellular heterogeneity and drug resistance. The prognosis of patients suffering from glioblastoma after 5 years is only 5%. In the present work, capsaicin analogues bearing modifications on the acyl chain with long-chain fatty acids showed promising anti-tumoral activity by its cytotoxicity on U-87 and U-138 glioblastoma multiforme cells. The capsaicin analogues were enzymatically synthetized with cross-linked enzyme aggregates of lipase B from Candida antarctica (CALB). The catalytic performance of recombinant CALB-CLEAs was compared to their immobilized form on a hydrophobic support. After 72 h of reaction, the synthesis of capsaicin analogues from linoleic acid, docosahexaenoic acid, and punicic acid achieved a maximum conversion of 69.7, 8.3 and 30.3% with CALB-CLEAs, respectively. Similar values were obtained with commercial CALB, with conversion yields of 58.3, 24.2 and 22% for capsaicin analogues from linoleic acid, DHA and punicic acid, respectively. Olvanil and dohevanil had a significant cytotoxic effect on both U-87 and U-138 glioblastoma cells. Irrespective of the immobilization form, CALB is an efficient biocatalyst for the synthesis of anti-tumoral capsaicin derivatives. biocatalyst for the synthesis of anti-tumoral capsaicin derivatives. Description: Artículo Sun, 01 Dec 2024 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1613 2024-12-01T00:00:00Z http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1609 Title: Biofunctionalization of natural extracts, trends in biological activity and kinetic release Authors: Martínez Olivo, Abraham Osiris; Zamora Gasga, Víctor Manuel; Medina Torres, Luis; Pérez Larios, Alejandro; Sáyago Ayerdi, Sonia Guadalupe; Sánchez Burgos, Jorge Alberto Abstract: Abstract: The health benefits provided by plant matrices is due to the presence of certain compounds that, in studies carried out in vitro and in vivo, have shown to have biological activity in certain conditions, not only as a natural treatment against various conditions, but also for the quality of preventing chronic diseases, these compounds, already identified and studied, they can increase their biological function by undergoing structural chemical modifications or by being incorporated into polymer matrices that allow, in the first instance, to protect said compound and increase its bioaccessibility, as well as to preserve or increase the biological effects. Although the stabilization of compounds is an important aspect, it is also the study of the kinetic parameters of the system that contains them, since, due to these studies, the potential application to these systems can be designated. In this review we will address some of the work focused on obtaining compounds with biological activity from plant sources, the functionalization of extracts through double emulsions and nanoemulsions, as well as their toxicity and finally the pharmacokinetic aspects of entrapment systems. Description: Artículo Tue, 01 Aug 2023 00:00:00 GMT http://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/1609 2023-08-01T00:00:00Z