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Title: Antinociceptive effects of Laelia anceps Lindl. and Cyrtopodium macrobulbon (Lex.) G.A. Romero & Carnevali, and comparative evaluation of their metabolomic profiles
Authors: Yáñez Barrientos, Eunice
González Ibarra, Alan Alexander
Wrobel, Kazimierz
Wrobel, Katarzyna
Corrales Escobosa, Alma Rosa
Alonso Castro, Ángel Josabad
Carranza Álvarez, Candy
Ponce Hernández, Amauri
Isiordia Espinoza, Mario Alberto
Ortiz Andrade, Rolffy
Torres Gutiérrez, Karen Lizbeth
Luna Rubio, Sarahí
Zapata Morales, Juan Ramón
Keywords: laelia anceps
cyrtopodium macrobulbon
nitric oxide
Issue Date: Mar-2022
Publisher: Elsevier
Citation: Yáñez-Barrientos E., González-Ibarra A.A., Wrobel K., Wrobel K., Corrales-Escobosa A.R., Alonso-Castro A.J., Carranza-Álvarez C., Ponce-Hernández A., Isiordia-Espinoza M.A., Ortiz-Andrade R, Torres-Gutiérrez K.L., Luna-Rubio S. & Zapata-Morales J.R. (2022). Antinociceptive effects of Laelia anceps Lindl. and Cyrtopodium macrobulbon (Lex.) G.A. Romero & Carnevali, and comparative evaluation of their metabolomic profiles. Journal of Ethnopharmacology, art. 115172. ISSN 0378-8741. DOI:
Series/Report no.: Journal of Ethnopharmacology;2022
Abstract: Abstract: Ethnopharmacological relevance Laelia anceps and Cyrtopodium macrobulbon are two orchids used in Mexican traditional medicine for treating pain. Aim of the study The individual antinociceptive activity of ethanol extracts from the roots of Laelia anceps (LAE) and Cyrtopodium macrobulbon (CME) was evaluated, and their metabolomic profiles were comparatively evaluated. The antinociceptive activity of CME and naproxen combination (1:1) was also addressed. Materials and methods The antinociceptive actions of LAE and CME were examined using three nociceptive tests. The combination of CME with naproxen was evaluated in the acetic acid test using isobologram analysis. Metabolomic analysis was performed using capillary reversed phase liquid chromatography–electrospray ionization-high resolution mass spectrometry and the MS-DIAL 4.70 software was used for data analysis and statistics. Results LAE (ED50 = 48.4 mg/kg) and CME (ED50 = 17.8 mg/kg) showed antinociceptive activity in the acetic acid test. Pre-treatment with L-NAME reverted the antinociceptive effects of LAE and CME in the acetic acid test. LAE (ED50 = 97 mg/kg) and CME (ED50 = 29 mg/kg) also induced antinociceptive activity in the second phase of the formalin test. The combination of CME with naproxen induced synergistic (interaction index = 0.434) antinociceptive effects (ED50 = 10.6 mg/kg). Overall, 156 compounds allocated in 97 different ontologies were found to be differentially expressed in the two orchids; among them, 125 compounds corresponded to LAE and 31 to CME. Three phenanthrene derivatives annotated in CME might be associated with its antinociceptive activity. Conclusion LAE and CME induced antinociceptive activity with the possible participation of the nitric oxide pathway. CME in combination with naproxen synergistically produces antinociceptive effects in the acetic acid test. The untargeted metabolomic analysis allowed for annotation of several compounds potentially involved in the therapeutic potential of two plants; among them, three phenanthrene derivatives might contribute to the observed antinociceptive activity.
Description: Artículo
ISSN: 0378-8741
Appears in Collections:3209 Artículos

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