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Title: Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
Authors: Rodríguez Razón, Christian Martín
Yáñez Sánchez, Irinea
Ramos Santillán, Vicente O
Velásquez Ordóñez, Celso
Gutiérrez Rubio, Susan A
García García, Maritza R.
López Roa, Roció I
Sánchez Hernández, Pedro Ernesto
Daneri Navarro, Adrián
García Iglesias, Trinidad
Keywords: silver nanoparticles
breast cancer
Issue Date: Feb-2018
Publisher: Dovepress
Citation: Rodríguez-Razón CM, Yañez-Sánchez I, Ramos-Santillan VO, Velásquez-Ordóñez C, Gutiérrez-Rubio SA, García-García MR, López-Roa RI, Sánchez-Hernández PE, Daneri-Navarro A, García-Iglesias T. Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis. Int J Nanomedicine. 2018;13:1081-1095
Series/Report no.: International Journal of Nanomedicine;Volume 13, Pages 1081 - 1095
Abstract: Background: Silver nanoparticles (AgNPs) have attracted considerable attention due to the variety of their applications in medicine and other sciences. AgNPs have been used in vitro for treatment of various diseases, such as hepatitis B and herpes simplex infections as well as colon, cervical, and lung cancers. In this study, we assessed the effect on proliferation, adhesion, and apoptosis in breast cancer cell lines of different molecular profiles (MCF7, HCC1954, and HCC70) exposed to AgNPs (2–9 nm). Methods: Breast cancer cell lines were incubated in vitro; MTT assay was used to assess proliferation. Adhesion was determined by real-time analysis with the xCELLingence system. Propidium iodide and fluorescein isothiocyanate-Annexin V assay were used to measure apoptosis. The transcriptome was assessed by gene expression microarray and Probabilistic Graphical Model (PGM) analyses. Results: The results showed a decreased adhesion in breast cancer cell lines and the control exposed to AgNPs was noted in 24 hours (p≤0.05). We observed a significant reduction in the proliferation of MCF7 and HCC70, but not in HCC1954. Apoptotic activity was seen in all cell lines exposed to AgNPs, with an apoptosis percentage of more than 60% in cancer cell lines and less than 60% in the control. PGM analysis confirmed, to some extent, the effects of AgNPs primarily on adhesion by changes in the extracellular matrix. Conclusion: Exposure to AgNPs causes an antiproliferative, apoptotic, and anti-adhesive effect in breast cancer cell lines cultured in vitro. More research is needed to evaluate the potential use of AgNPs to treat different molecular profiles of breast cancer in humans.
Description: Artículo
ISSN: 1176-9114 Print
1178-2013 Online
Appears in Collections:3209 Artículos

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