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Title: DNA oxidative damage in oral cancer: 8-hydroxy-2´-deoxyguanosine immunoexpression assessment
Authors: Prieto Correa, José Roberto
Bologna Molina, Ronell Eduardo
González González, Rogelio
Molina Frechero, Nelly
Soto Ávila, Juan José
Isiordia Espinoza, Mario Alberto
Barrón Márquez, Mariana Cristina
López Verdín, Sandra
Keywords: DNA oxidative damage
oral squamous cell carcinoma
free radical
Issue Date: Jun-2023
Publisher: PubMed
Citation: Prieto-Correa JR, Bologna-Molina R, González-González R, Molina-Frechero N, Soto-Ávila JJ, Isiordia-Espinoza M, et al. DNA oxidative damage in oral cancer: 8-hydroxy-2´-deoxyguanosine immunoexpression assessment. Med Oral Patol Oral Cir Bucal. 2023. doi:10.4317/medoral.25924
Series/Report no.: Medicina oral, Patología oral y Cirugía bucal;2023 in press
Abstract: Abstract Background: The development and establishment of oral squamous cell carcinoma are confined to carcinogenesis, which involves oxidative stress via oxygen-free radical production as a hydroxyl radical (HO•), considered the most important cause of oxidative damage to basic biomolecules since it targets DNA strands. 8-Hydroxy-2´-deoxyguanosine (8-OHdG) is considered a free radical with a promutagenic capacity due to its ability to pair with adenosine instead of cytosine during replication. Material and methods: We collected 30 paraffin-embedded tissue samples of OSCC from patients treated between 2013 and 2018. We recorded risk habits, disease stage, disease free survival and death with at least 3 years of follow-up. 8-Hydroxyguanosine was evaluated by immunohistochemistry and subsequently classified as weak-moderate or strong positive expression. Additionally, we noted whether it was expressed in the cytoplasm and/or nucleus. Results: Most of the cases expressed 8-OHdG with a strong intensity (80%). All neoplastic cells were preferentially stained in only the cytoplasm (70.0%), but nuclear positivity was found in 30%, independent of the intensity. Based on the location in the cytoplasm and/or nucleus, tumors >4 cm showed a high frequency (95.5%) of 8-OHdG expression in only the cytoplasm, with a significant difference (p value 0.001). Additionally, overall survival was affected when immunoexpression was present in the cytoplasm and nucleus because all deaths were in this group were statistically significant (p value = 0.001). Conclusions: All tumors showed DNA oxidative damage, and 8-OHdG was preferentially expressed in the cytoplasm. This finding was associated with tumor size and, when present in the nucleus, might also be related to death.
Description: Artículo
ISSN: 1698-6946 online
Appears in Collections:3209 Artículos

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