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Title: In Vitro Conservation of Mexican Garlic Varieties by Minimal Growth
Authors: Tirado, Bibiana
Gómez Rodríguez, Víctor Manuel
Cruz Cárdenas, Carlos Iván
Zelaya Molina, Lily Xochilt
Ramírez Vega, Huberto
Sandoval Cancino, Gabriela
Keywords: garlic
genetic resources
in vitro conservation
osmotic agents
Issue Date: Dec-2023
Publisher: MDPI
Citation: Tirado, B.; GómezRodríguez, V.M.; Cruz-Cárdenas, C.I.; Zelaya-Molina, L.X.; Ramírez-Vega, H.; Sandoval-Cancino, G. In Vitro Conservation of Mexican Garlic Varieties by Minimal Growth. Plants 2023, 12, 3929. 10.3390/plants12233929
Series/Report no.: Plants;2023, Vol.12, Issue 23. 3929
Abstract: Garlic (Allium sativum L.) is one of the 30 crops that are essential for world food; therefore, its conservation should be considered a priority. There are two main plant conservation strategies: in situ and ex situ conservation. Both strategies are important; nevertheless, ex situ field conservation is affected by biotic and abiotic factors. A complementary strategy to preserve garlic germplasm in the medium term is through in vitro culture by minimal growth. The aim of this study was to evaluate the in vitro conservation of three Mexican garlic varieties by minimal growth. Garlic plants obtained from in vitro garlic bulbs were preserved in six culture media at 25, 18, and 5 °C. A randomized design was used and an analysis of the variance of the survival, contamination, and shoot height of the explants was performed at 30, 60, 90, 180, 270, and 365 days of culture. The results showed that the in vitro conservation of Pebeco, Tacátzcuaro Especial, and Huerteño garlic varieties was optimally obtained for one year at 5 °C in a basal Murashige and Skoog (MS) culture medium with 68.46 g L−1 sucrose and 36.43 g L−1 sorbitol. Thus, the achieved protocol can be adapted to other varieties of garlic for medium-term storage in germplasm banks.
Description: Artículo
ISSN: 2223-7747
Appears in Collections:2403 Artículos

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