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Title: Physical mapping of 5S and 18S ribosomal DNA in three species of Agave (Asparagales, Asparagaceae)
Authors: Gómez Rodríguez, Víctor Manuel
Rodríguez Garay, Benjamín
Palomino, Guadalupe
Martínez, Javier
Barba González, Rodrigo
Keywords: agave
fluorescent in situ hybridization
ribosomal DNA
physical mapping
Issue Date: 12-Aug-2013
Publisher: Comparative Cytogenetics. International Journal of Plant & Animal Cytogenetics, Karyosystematics, and Molecular Systematics.
Citation: Gomez-Rodriguez VM, Rodriguez-Garay B, Palomino G, Martínez J, Barba-Gonzalez R (2013) Physical mapping of 5S and 18S ribosomal DNA in three species of Agave (Asparagales, Asparagaceae). Comparative Cytogenetics 7(3): 191–203. doi: 10.3897/CompCytogen.v7i3.5337
Series/Report no.: Comparative Cytogenetics;7(3): 191–203 (2013)
Abstract: Agave Linnaeus, 1753 is endemic of America and is considered one of the most important crops in Mexico due to its key role in the country’s economy. Cytogenetic analysis was carried out in A. tequilana Weber, 1902 ‘Azul’, A. cupreata Trelease et Berger, 1915 and A. angustifolia Haworth, 1812. The analysis showed that in all species the diploid chromosome number was 2n = 60, with bimodal karyotypes composed of five pairs of large chromosomes and 25 pairs of small chromosomes. Furthermore, different karyotypical formulae as well as a secondary constriction in a large chromosome pair were found in all species. Fluorescent in situ hybridization (FISH) was used for physical mapping of 5S and 18S ribosomal DNA (rDNA). All species analyzed showed that 5S rDNA was located in both arms of a small chromosome pair, while 18S rDNA was associated with the secondary constriction of a large chromosome pair. Data of FISH analysis provides new information about the position and number of rDNA loci and helps for detection of hybrids in breeding programs as well as evolutionary studies.
ISSN: 1993-078X
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